Improvement of yields and rates during enzymatic hydrolysis of cellulose to glucose

by Donald W. Sundstrom

Publisher: Dept. of Energy, Publisher: for sale by the National Technical Information Service] in [Washington], [Springfield, Va

Written in English
Published: Downloads: 275
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Subjects:

  • Cellulose,
  • Hydrolysis,
  • Enzymes -- Synthesis,
  • Glucose

Edition Notes

StatementD. W. Sundstrom, H. E. Klei, R. W. Coughlin, University of Connecticut
SeriesCOO ; 5141-1
ContributionsKlei, Herbert E., 1935- joint author, Coughlin, Robert W., 1934- joint author, University of Connecticut, United States. Dept. of Energy
The Physical Object
Paginationv. :
ID Numbers
Open LibraryOL14877885M

Donald W. Sundstrom has written: 'Improvement of yields and rates during enzymatic hydrolysis of cellulose to glucose' -- subject(s): Cellulose, Enzymes, Glucose, Hydrolysis, Synthesis. High yields of sugars via the non-enzymatic hydrolysis of cellulose V. Berberi1, F. Turcotte1, G. Lantagne2, M. Chornet1,3 & J.-M. Lavoie1 1Département de Génie Chimique, Université de Sherbrooke, Canada 2Institut de recherche d'Hydro-Québec (IREQ), Canada 3CRB Innovations Inc., Canada Abstract Given the cost of cellulosics (quasi-homogeneous residual feeds range in North. Download Citation | Effects of different pretreatment methods on the enzymatic hydrolysis of cassava residue | The enzymatic hydrolysis of cassava residue treated by a hot water (HW) pretreatment.   Similar to results from previous studies [9,10,22,26], the enzymatic hydrolysis yield for the cellulose samples pretreated with pure [AMIM]Cl (χ [AMIM]Cl = ) was significantly increased; the highest hydrolysis yield was % after 72 hours, which was times that of the hot-water-treated cellulose and times that of the original.

During hydrolysis anhydrous form will be changed to monosaccharide, which increased the weight. That is the reason for in cellulose or glucomannan (hexose) and in xylose or arabinose. cellulose–enzyme complexes and change during the process. The rate decrease in the course of the process is determined not only by the pre-exponential factor decrease but by the enzyme inhibition as well. Keywords: bleached hardwood pulp, enzyme hydrolysis, kinetics. INTRODUCTION The enzyme hydrolysis of cellulose to glucose. product of cellulose hydrolysis (that is, other than cellobiose). The only exceptions have been those attributable to substituted sugars resulting from hydrolysis of CMC, cellulose sulfate, or other substi-tutedcelluloses (Levinsonet al., ). "Intermediates" were detected by Kooiman et at. () during the enzymatic hydrolysis of cellulose. Improvement of yields and rates during enzymatic hydrolysis of cellulose to glucose. Final report, December 1, Janu Technical Report Sundstrom, D W ; Klei, H E ; Coughlin, R W.

  The mass balance around the enzymatic hydrolysis step achieved % and % closure for glucose and xylose, respectively. In enzymatic hydrolysis at % solids loading, % of the total input sugars (glucose and xylose) were hydrolyzed and solubilized, of which, % was hydrolyzed to their monomers (Fig. 5, Table S3). About two-thirds. In the absence of this enzyme, glucosephosphate cannot be converted to glucose and instead accumulates in the liver and is converted to glucosephosphate, which is used for glycogen synthesis. Glycogen synthesis is therefore elevated in the livers of patients with this disease. Stable glucose concentrations were achieved, and BSA gave 30 to 40% higher glucose yields for continuous hydrolysis of both pure cellulose and pretreated biomass. To better understand BSA effects, enzymatic hydrolysis of Avicel was interrupted over nearly complete hydrolysis using our previously developed restart protocol. obstruction for cellulose to be hydrolyzed into glucose, and the glucose yield would become lower. In order to eliminate the negative influence of cellobiose on the system of enzymatic hydrolysis, cellobiase was added into the hydrolysate. Figure 1 shows the effect of cellobiase on the release of glucose from enzymatic hydrolysis at 2% (w/v.

Improvement of yields and rates during enzymatic hydrolysis of cellulose to glucose by Donald W. Sundstrom Download PDF EPUB FB2

The enzymatic hydrolysis of the IL-treated products resulted in higher glucose and xylose saccharification yields than when no treatment was employed. [Emim] [Ac] was found to be the most efficient IL resulting in glucose and xylose yields of % and %, respectively, after a h saccharification by:   In comparison, the 72 hour hydrolysis yield with an enzyme loading of mg EP/g glucose had an enzymatic productivity of g glucose/mg enzyme protein, and a 95% glucose yield with a mg EP/g cellulose enzyme loading would Improvement of yields and rates during enzymatic hydrolysis of cellulose to glucose book a productivity of g glucose/mg enzyme.

The average glucose yield from enzymatic hydrolysis was up to mg/g pretreated biomass and was higher than that from acid hydrolysis (the maximum yield was mg/g extracted biomass). As a result of the LHW pretreatment, up to % of the hemicelluloses were removed from the solid fraction.

Interestingly, cellulose III has been shown to have fold higher rate of enzymatic hydrolysis compared to native cellulose I (isolated from Cladophora algae cell walls). The extent of cellulose III formation during AFEX (and related ammonia-based pretreatments) treatment of lignocellulosic biomass is being currently investigated (Chundawat.

Cellulose is difficult to degrade due to its complex and highly crystalline structure. Hydrolysis of cellulose is a crucial step in the overall bioethanol production pathway due to its large cost contribution, mainly because of much higher amount of enzyme usage (40 to times than that of starch hydrolysis) (Merino and Cherry,Wang et al., ).Cited by: 3.

Breaking down the cellulose into sugars, predominantly glucose, can pose a challenge. One way to do so is by enzymatic hydrolysis. This uses enzymes, biological catalysts, to break down the cellulose polymers into monomeric sugars. Enzymatic hydrolysis of cellulosic biomass is a key obstacle that must be overcome in.

Because efficient hemicellulose decomposition and removal improved access by the enzyme to the cellulose during enzymatic hydrolysis, the maximum glucose yield was observed to be mg/g-Eu at a solid concentration of 50 wt% and SF ofwithout the need for any additional physical and/or chemical pretreatment.

Declarations of interest. None. Cellulose hydrolysis was further assessed by using different concentrations of Avicel® (15–40 g L −1) in seawater (1X).Results are depicted in Fig. observed, glucose was formed at rates of ∼ ± g glucose L −1 h −1 under Avicel® loading conditions ranging from 20 to 30 g L − 40 g L −1 loadings, however, hydrolytic performance was lower than in the other cases.

differently to surfactants during the hydrolysis of cellulose Chia-wen C Hsieh1*, David Cannella1, Henning Jørgensen2, Claus Felby1 and Lisbeth G Thygesen1 Abstract Background: Non-ionic surfactants such as polyethylene glycol (PEG) can increase the glucose yield obtained from enzymatic saccharification of lignocellulosic substrates.

Carbon catalysts based on modified Sibunit material was used for the first time for cellulose hydrolysis-dehydration. The yields of glucose (up to 74 % in a flow reactor) and 5-HMF (up to 21 % in.

The enzymatic saccharification of partially delignified cellulignin (PDCL) of sugar cane bagasse was studied to verify how hydrolysis yield and hydrolysis rate were affected by the lignin content. Results show that achievable glucose yields and the enzyme dosages required to reach them vary widely between the enzyme preparations and also depend on hydrolysis mode, with better performance observed on washed PCS than on WS PCS.

In SSF mode, all four enzyme systems achieved glucose yields from cellulose of 90% of theoretical or higher. hydrolysis rate (Chang et al. ; Draude et al. To loosen the structure and improve access of enzymes, the cellulose substrates were treated with concentrated alkali solutions, liquid ammonia, amines, and other activating agents.

It was found that activation of cellulose structure accelerates the enzymatic hydrolysis (Igarishi et al. (S), while the degradation of cellulose to glucose (G) is accomplished in the next step, enzymatic hydrolysis. This step is framed as the focus of this thesis will be on the enzymatic hydrolysis of cellulose.

The recent discovery of accessory proteins that boost cellulose hydrolysis has increased the economical and technical efficiency of processing cellulose to bioethanol. Oxidative enzymes (e.g. GH61) present in new commercial enzyme preparations have shown to increase cellulose conversion yields.

When using pure cellulose substrates it has been determined that both oxidized and. The enzymatic hydrolysis of lignocellulosic biomass is known to be product-inhibited by glucose. In this study, the effects on cellulolytic glucose yields of glucose inhibition and in situ glucose.

Enzymatic biomass saccharification is an important process for bioethanol production. Hitherto, numerous cellulase cocktails (crude enzyme) have been developed to improve enzymatic activity.

For this purpose, the synergy of incorporating hydrolase functionality within a cellulase cocktail is a key function. However, such synergistic action, by potentially numerous different enzyme types, on. Product yields for the hydrolysis of cotton cellulose at K for 60 min in wt% aqueous formic acid, wt% acetic acid solution, and water alone are listed in Table 1.

The addition of the acids was effective for significantly increasing the yields of glucose and oligomers with lower degrees of polymerization.

Selig et al. achieved an 84 % improvement in the enzymatic hydrolysis of hot water pretreated corn stover by adding an endoxylanase, a ferulic acid esterase and an acetyl xylan esterase to a the cellobiohydrolase Cel7A. These authors also observed that the resulting synergistic effect is more evident when low Cel7A loadings are used.

When glucose was removed by dialysis during the enzymatic hydrolysis, the cellulose conversion rates and glucose yields increased. In fact, with dialytic in situ glucose removal, the rate of enzyme-catalyzed glucose release during 48–72 h of reaction recovered from 20–40% to become ≈70% of the rate recorded during 6–24 h of reaction.

productivity (Alvira et al. In order to improve the yield and rate of the enzymatic hydrolysis and reduce enzyme costs, research among others has focused on enhancement of cellulase productivity and optimization of cellulose hydrolysis (Ferreira et al. ; Sun and Cheng ).

Trichoderma reesei (Kansoh et al. ; Eveleigh et al. More than times improvement in glucose yield was observed with 10 wt% NaOH and 4 h of sonication, compared to untreated samples.

The glucose yields increased with increase in initial particle size of cellulose, while DP had no effect on glucose yields. At the optimal conditions of pH ofsolids loadings of 10% and enzyme loadings of 20 FPU/g DM, the enzymatic hydrolysis of pretreated PCG produced a glucose yield of % from the maximum yield which represents an increase of 15% over the non-optimized controls at the zero-level central points.

Cellulose from the Gram-negative bacterium Acetobacter xylinum has been used as a model substrate for visualizing the action of cellulase enzymes from the fungus Trichoderma reesei. High-resolution electron microscopy reveals that A. xylinum normally produces a ribbon of cellulose that is a composite of bundles of crystalline microfibrils.

Visual patterns of the process of cellulose. Glucose production rates (GPRs) during 4-h enzymatic hydrolysis of lignocellulose substrates.

a Reed canary grass; and b Pine bark. Enzymatic hydrolysis experiments were performed with either non-treated or H 2 SO 4-treated (SF ), or (S)IL-treated lignocelluloses. (S)IL treatments were performed at (A) SF (B) SF and (C) SF In this article we will discuss about the breakdown and synthesis of: 1.

Sucrose 2. Starch and 3. Cellulose in Plants. Breakdown of Sucrose: Sucrose is broken down or hydrolysed to yield glucose and fructose in the presence of the enzyme invertase or sucrase. Enzymatic hydrolysis of cellulose was carried out in 50 mM citrate buffer (pH ) containing % (w/v) microcrystalline cellulose (Avicel PH, Sigma).

Celluclast L (60 FPU/mL) and β-glucosidase ( IU/mL, Novozyme ) were added at FPU/g-cellulose and IU/g-cellulose, respectively. Cellulose yields D-glucose after complete acid hydrolysis, yet humans are unable to metabolize cellulose as a source of glucose.

Our digestive juices lack enzymes that can hydrolyze the β-glycosidic linkages found in cellulose, so although we can eat potatoes, we cannot eat grass. Enzymatic hydrolysis is a key step in the bioconversion of cellulose to ethanol, and the focus of our research was to improve the yield and rate of enzymatic hydrolysis.

Xylanse is known to have a synergetic effect on cellulose hydrolysis by degrading heterogenous xylan polymer that surrounds cellulose microfibrils [ 14 ].

The enzymatic hydrolysis of cellulose encounters various limitations that are both substrate‐ and enzyme‐related. Although the crystallinity of pure cellulosic Avicel plays a major role in determining the rate of hydrolysis by cellulases from Trichoderma reesei, we show that it stays constant during enzymatic mode of action of cellulases was investigated by studying their.

Shen et al. found that the glucan-glucose conversion yield increased significantly when β-glucosidase was supplemented during the enzymatic hydrolysis of steam-pretreated sweet sorghum bagasse with SO 2. Furthermore, results showed that the increase of β-glucosidase dosage from 5 to 10 U/g could improve the hydrolysis yield from to %.The yield of substrate without pretreatment for glucose after 24 hour incubation was only % of the dry substrate (Table.

3). Table 3. Enzymatic hydrolysis results without pretreatment Sugar yield (g / kg of dry substrate) Glucose Xylose Galactos Mannitol Mannose Fructose Arabinose. 0 conditions (temperature, pH, etc.).

To improve the yield and rate of the enzymatic hydrolysis, optimizing the hydrolysis process and enhancing cellulase activity need to be focused (Cantwell et al.

). During the enzymatic hydrolysis, cellulose is depoly-merized by .